Anti-toxic principles from Moringa oleifera and Musa sapientium down-regulated Ki67 and Multidrug resistance1 proteins in Cadmium Chloride-induced hepato-toxicity and mutagenesis in rats.

Abstract

Moringa oleifera (MO) and Musa sapientium (MS) are ethno-medicinal plants with anticancer potentials. Cadmium is a pro-carcinogen of global health concerns. This study evaluated the anticancer potentials of MOF6 (extracted from MO leaves) and MSF1 (extracted from MS suckers) on immuno-modulations of Ki67 (proliferation biomarker) and Multi-drug resistance1 (MDR1) proteins in the liver of rats in Cadmium Chloride (CdCl)-induced hepato-toxicity and mutagenesis. 55 adult male rats were randomly divided into 11 groups (n = 5). Group 1 received physiological saline. Groups 2-6 and 10-11 received single intra-peritoneal administration of 1.25mg/kg bodyweight of CdCl on Day 1. Groups 3-5 were treated with 15 and 30mg/kg bodyweight of MOF6,and 10mg/kg bodyweight of MSF1 respectively from Days 15-56. Group 6 was treated with 3.35 mg/kg bodyweight of Doxorubicin and intravenous injection of 0.5ml/200g of Cisplatin from Days 15-29. Groups 7-9 received only 15 and 30mg/kg bodyweight of MOF6, and 10mg/kg bodyweight of MSF1 respectively from Days 1-56. Groups 10 and 11 received preventive treatments with 30mg/kg bodyweight of MOF6 and 10mg/kg bodyweight of MSF1 respectively from Days 1-56. Doxorubicin and extracts doses were administered orally. Consequently, Ki67 and MDR1 concentrations in Liver homogenates were evaluated using Enzyme Linked Immunosorbent Assay. Computed data were statistically analyzed (p≤0.05). Results showed statistically significant (p≤0.05) and non-significant decreased concentrations (p≥0.05) of Ki67 and MDR1 in Groups 3-11 comparedwith Group 2. Therefore, MOF6 and MSF1 ameliorated CdCl-induced hepato-toxicity, mutagenesis,hyperplasia and drug resistance. In conclusion, MOF6 and MSF1 possess hepato-protective, antiproliferation, anti-drug resistance and anticancer potentials.