Comparative analysis of de novo assembly of Bacillus cereus HUB4-4 short reads

Abstract

The automated Sanger method is considered as a first generation technology and newer methods are referred to as next generation sequencing. These newer technologies constitute various strategies that rely on a combination of template preparation sequencing and imaging and genome alignment and assembly methods. NGS or high throughput sequencing technologies are intended to lower the cost of DNA sequencing beyond what is possible with standard dye terminator methods. Bacillus cereus is a very common dirt-dwelling bacterium which can survive in an oxygen environment with the help of protective endospores. There are several strains like HUB4-4,HUB2-3, CER057etc are cause harm to humans, whereas others are used as helpful probiotics. De novo assembly is mainly used for the purpose of assembling short reads to create full-length sequences. Bacillus cereus HUB4-4 for this organism there is no contigs and scaffolds are available. So De novo assembly was carried out to find scaffolds and contigs by using the CLC Genomics Workbench and DRA pipeline and N25, N50, N75 contigs were determined. For identified N50 contigs, BLAST is done at NCBI and identified related organism toBacillus cereus HUB4-4 in CLC genomics. N50 contigs in DRA pipeline were also determined by using three software tools like soap de novo, velvet, abyss for the Bacillus cereus HUB4-4 data using short read assembly for paired end data and compare the output of these two tools and identified CLC genomics workbench is more efficient.