Formulation, Evaluation and Validation of Newly Formulated Laportea Arishta

Abstract

Laportea Arishtas are self generated herbal fermentations made with decoctions of Laportea interrupta leaves. The improvement in the extraction of drug molecule from the herb, improvement in drug delivery into the human body sites and enhancing therapeutic properties of the herb are the main reason for selecting this herb. Physico chemical analysis was performed for the crude drug for assuring its authenticity. The formulated arishta were subjected to organoleptic-physicochemical analysis, qualitative and quantitative analysis, antioxidant studies along with a new analytical method validation. The quantitative estimations were performed by determining the alcoholic, phenolic, flavanoid contents along with reducing sugar determination. In vitro antioxidant activity was performed by FTC, FRAP and TBA method. Validation experiments of new Laportea arishta were performed to demonstrate linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ as per ICH guidelines