Comparative Assessment of Serum Protein Profiles in Broiler and Indigenous Chickens in Response to Different Feeding Patterns

Abstract

Although the binding of antibiotics to serum proteins has been extensively investigated, limited information is available on how antibiotics influence serum protein levels under different feeding patterns. This study evaluated the effects of enrofloxacin and colistin on serum proteins (albumin and globulin) in broiler and indigenous chickens fed either commercial or organic diets. A total of 80 one-day-old chicks were assigned to enrofloxacin (n = 40) and colistin (n = 40) treatment groups. Each treatment included 20 broiler and 20 indigenous chicks, which were further allocated into experimental and control groups. Antibiotics were administered through drinking water over a 4-week experimental period. Blood samples were collected weekly, serum was separated by centrifugation, and albumin and globulin concentrations were determined using standard laboratory methods. In all control groups, serum protein levels increased progressively throughout the study. In contrast, antibiotic-treated groups exhibited a reduction in albumin and globulin concentrations, although protein levels remained unchanged during the fourth week in some groups. Overall, both enrofloxacin and colistin reduced serum protein levels in broiler and indigenous chickens regardless of whether they were fed commercial or organic diets, indicating that antibiotic treatment adversely affects serum protein metabolism during the growth period.